| # | Seed | Team | Region | Conf | Bid |
|---|---|---|---|---|---|
| 1 | 1 | Duke | East | ACC | Auto |
| 2 | 1 | Illinois | West | Big Ten | Auto |
| 3 | 1 | Florida | South | SEC | Auto |
| 4 | 1 | Houston | Midwest | Big 12 | Auto |
| 5 | 2 | Kansas | Midwest | Big 12 | At-large |
| 6 | 2 | UConn | South | Big East | Auto |
| 7 | 2 | Michigan State | West | Big Ten | At-large |
| 8 | 2 | Texas Tech | East | Big 12 | At-large |
| 9 | 3 | Nebraska | East | Big Ten | At-large |
| 10 | 3 | Texas | West | SEC | At-large |
| 11 | 3 | Iowa State | South | Big 12 | At-large |
| 12 | 3 | Vanderbilt | Midwest | SEC | At-large |
| 13 | 4 | Marquette | Midwest | Big East | At-large |
| 14 | 4 | Michigan | South | Big Ten | At-large |
| 15 | 4 | BYU | West | Big 12 | At-large |
| 16 | 4 | Missouri | East | SEC | At-large |
| 17 | 5 | Virginia | East | ACC | At-large |
| 18 | 5 | UCLA | West | Big Ten | At-large |
| 19 | 5 | Auburn | South | SEC | At-large |
| 20 | 5 | Alabama | Midwest | SEC | At-large |
| 21 | 6 | Miami | Midwest | ACC | At-large |
| 22 | 6 | Tennessee | South | SEC | At-large |
| 23 | 6 | TCU | West | Big 12 | At-large |
| 24 | 6 | Louisville | East | ACC | At-large |
| 25 | 7 | Oklahoma State | East | Big 12 | At-large |
| 26 | 7 | Gonzaga | West | Pac-12 | Auto |
| 27 | 7 | Arizona | South | Big 12 | At-large |
| 28 | 7 | Baylor | Midwest | Big 12 | At-large |
| 29 | 8 | USC | Midwest | Big Ten | At-large |
| 30 | 8 | Iowa | South | Big Ten | At-large |
| 31 | 8 | West Virginia | West | Big 12 | At-large |
| 32 | 8 | Arkansas | East | SEC | At-large |
| 33 | 9 | NC State | East | ACC | At-large |
| 34 | 9 | St. John's | West | Big East | At-large |
| 35 | 9 | Cincinnati | South | Big 12 | At-large |
| 36 | 9 | Ole Miss | Midwest | SEC | At-large |
| 37 | 10 | Texas A&M | Midwest | SEC | At-large |
| 38 | 10 | Kentucky | South | SEC | At-large |
| 39 | 10 | Utah StateOpening | West | Pac-12 | At-large |
| 40 | 10 | VillanovaOpening | West | Big East | At-large |
| 41 | 10 | Virginia TechOpening | East | ACC | At-large |
| 42 | 10 | LSUOpening | East | SEC | At-large |
| 43 | 11 | Mississippi StateOpening | East | SEC | At-large |
| 44 | 11 | Ohio StateOpening | East | Big Ten | At-large |
| 45 | 11 | Saint Louis | West | A-10 | Auto |
| 46 | 11 | IndianaOpening | South | Big Ten | At-large |
| 47 | 11 | XavierOpening | South | Big East | At-large |
| 48 | 11 | PurdueOpening | Midwest | Big Ten | At-large |
| 49 | 11 | SyracuseOpening | Midwest | ACC | At-large |
| 50 | 12 | WisconsinOpening | Midwest | Big Ten | At-large |
| 51 | 12 | North CarolinaOpening | Midwest | ACC | At-large |
| 52 | 12 | New Mexico | South | Mountain West | Auto |
| 53 | 12 | Saint Mary's | West | WCC | Auto |
| 54 | 12 | Memphis | East | American | Auto |
| 55 | 13 | High Point | East | Big South | Auto |
| 56 | 13 | UIC | West | MVC | Auto |
| 57 | 13 | UC San Diego | South | Big West | Auto |
| 58 | 13 | Akron | Midwest | MAC | Auto |
| 59 | 14 | Wright State | Midwest | Horizon | Auto |
| 60 | 14 | McNeese | South | Southland | Auto |
| 61 | 14 | Montana State | West | Big Sky | Auto |
| 62 | 14 | Yale | East | Ivy | Auto |
| 63 | 15 | Western Kentucky | East | CUSA | Auto |
| 64 | 15 | Furman | West | SoCon | Auto |
| 65 | 15 | CharlestonOpening | South | CAA | Auto |
| 66 | 15 | Arkansas StateOpening | South | Sun Belt | Auto |
| 67 | 15 | Central ArkansasOpening | Midwest | UAC | Auto |
| 68 | 15 | South Dakota StateOpening | Midwest | Summit | Auto |
| 69 | 16 | American UniversityOpening | Midwest | Patriot | Auto |
| 70 | 16 | JacksonvilleOpening | Midwest | ASUN | Auto |
| 71 | 16 | Bethune-CookmanOpening | South | SWAC | Auto |
| 72 | 16 | SienaOpening | South | MAAC | Auto |
| 73 | 16 | HowardOpening | West | MEAC | Auto |
| 74 | 16 | VermontOpening | West | America East | Auto |
| 75 | 16 | Southeast Missouri StateOpening | East | OVC | Auto |
| 76 | 16 | Long Island UniversityOpening | East | NEC | Auto |